The HDZip Class I Transcription Factors in - DiVA portal

En aba-ökad interaktion mellan pyl6 aba-receptorn med myc2

Se hela listan på frontiersin.org Overexpression of ABI5 Confers ABA Sensitivity to hy5 and Inhibits Hypocotyl Elongation. If ABI5 is a direct target of HY5, one would expect that overexpression of ABI5 may be able to rescue some of the hy5 mutant phenotypes such as its reduced sensitivity to ABA. The ABI5 overexpression lines were generated in both Col-0 and hy5 background 2020-04-11 · Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development. It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated. 2019-06-01 · However, the overexpression of ABI5 in the bes1-D background could not rescue the early-flowering phenotype . Because plants overexpressing ABI5 decreased the ABA insensitivity of bes1-D , it is indicated that ABI3-mediated negative regulation of the floral transition would be independent of ABI5 activity, at least under higher-BR-response conditions. We have shown that both ABI5 and ABI3 overexpression can increase endogenous ABI5 protein levels in vivo in a concentration dependent manner (Figures 2e and 3a).

Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination.

En aba-ökad interaktion mellan pyl6 aba-receptorn med myc2

S2 a,b). Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5.

Wrky proteiner: signalering och reglering av uttryck under abiotiska

The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay ﬂowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating ﬂowering time is unknown. Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state.

Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). ABI5 in the hy5 mutant background restored its ABA sensitivity and overexpression of ABI5 in the wild-type background-enhanced light response. These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5. Results HY5 Mediates ABA Response in Roots and Is Required for Osmotic The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked.
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Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002). Analyses of transcript accumulation in abi5 mutants suggest that, similar to ABI3, ABI5 has both activator and repressor functions that may have either synergistic or antagonistic effects on gene expression, depending on the target gene.

AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress.
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En aba-ökad interaktion mellan pyl6 aba-receptorn med myc2

Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line. (A) GUS staining of leaves of 7-day-old ABI5-GUS OE12 plants treated with DMSO, ABA (50 μM), and AZD (2 μM) for 48 h.

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The HDZip Class I Transcription Factors in - DiVA portal

Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants.